Laser Scanning Confocal Microscope Magnification

Laser scanning confocal microscopy laser scanning confocal microscopes employ a pair of pinhole apertures to limit the specimen focal plane to a confined volume approximately a micron in size.

Laser scanning confocal microscope magnification.

Capturing multiple two dimensional images at different depths in a sample enables the. The confocal laser scanning microscope clsm is a microscope which focuses only on a single focal plane and the unfocused plane will not be visualized. This means that we can view visual sections of tiny structures that. A confocal laser scanning microscope or also known as laser scanning confocal microscope is used to obtain high resolution images and generate 3d reconstructions through direct optical sectioning to provide clear images from a range of depths of thicker specimens for nanometer level imaging and measurement.

Relatively thick specimens can be imaged in successive volumes by acquiring a series of sections along the optical z axis of the microscope. Clsm combines high resolution optical imaging with depth selectivity which allows us to do optical sectioning. With confocal laser scanning microscopy clsm we can find out even more. The laser scans across the object and an image is built up pixel by pixel on a screen.

Confocal microscopy most frequently confocal laser scanning microscopy clsm or laser confocal scanning microscopy lcsm is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a spatial pinhole to block out of focus light in image formation.