When applied to plant cells and especially to woody tissues lcm requires extensive optimization to overcome such factors as rigid cell walls large central vacuoles intercellular spaces and technical issues with thickness and flatness of the sections. At the moment we are using a protocol with 24h formalin fixation and 24h decalcification but the achieved rna quality after laser capture microdissection is poor and also immunofluorescence. 1 department of biomedical research university of veterinary medicine vienna.
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